63ª Reunião Anual da SBPC

E. Ciências Agrárias - 1. Agronomia - 4. Fitotecnia

Carbohydrates and proteins in leaf of 14 common bean genotypes

Maria do Bom Conselho Lacerda Medeiros 1 Argemiro Pereira Martins Filho 1 Eldenira Barbosa Uchôa 1 Odyone Nascimento da Silva 1 Izabelle Pereira Andrade 1 Allan Klynger da Silva Lobato 1

1. Núcleo de Pesquisa Vegetal Básica e Aplicada, Univesidade Federal Rural da Amazônia

INTRODUÇÃO:

Carbon and nitrogen are major constituents of plant biomass, in which carbohydrates are fixed during photosynthesis and proteins are formed by amino acids after nitrate and/or ammonium absorption. Nutritionally, common bean grain is frequently used as source proteins and carbohydrates by Brazilian population, and this crop presents also economic importance for this country. Aim of this investigation was to obtain responses linked to carbon and nitrogen metabolisms of 14 genotypes of common bean favorable to Brazilian conditions, and it was evaluated total soluble carbohydrates and total soluble proteins.

METODOLOGIA:

Study was conducted in experimental area located in Centro de Treinamento em Irrigação (CTI) of Universidade Estadual de Maringá (UEM), during 2008 season. Fertilization was carried out based in soil analysis and crop exigency. Plants were grown in field conditions with minimum/maximum temperature of 15.2/32.7ºC, and relative humidity was 48/74%, respectively. Experimental design used was randomized blocks, with 14 genotypes (IAPAR 81, IPR Tangará, IPR Uirapuru, BRS Campeiro, CHC 9729, CHP 9859, GEN C2-1-1, GEN C2-1-3, SM 1007, SN 1207, CNFC 10408, CNCF 10429, LP 0403, and LP 0472). Experiment was composed of 4 blocks, and each plot with 5x5 m (diameter x width), being used spacing of 1 and 2 m among plots and blocks, respectively. Total soluble carbohydrates and total soluble proteins were determined according to Dubois et al. (1956) and Bradford (1976), respectively. In this study were evaluated total soluble carbohydrates and total soluble proteins. Data were analyzed employing a variance analysis, and using Scott-Knott test at 5% level of probability. Standard errors were also calculated in all treatments evaluated.

RESULTADOS:

Results showed in total soluble carbohydrates levels indicated that this parameter is significantly different in 14 genotypes studded, as well as higher level was obtained in BRS Campeiro genotype. In intermediate group were showed values fluctuating between 1.25 and 1.16 mmol g DM-1, and it was composed by IAPAR 81, CHP 9859, SM 1007, and LP 0403 genotypes. Group formed by IPR Tangará, IPR Uirapuru, CHC 9729, GEN C2-1-1, GEN C2-1-3, SN 1207, CNFC 10408, CNFC 10429, and LP 0472 genotypes oscillated between 0.97 and 1.12 mmol g DM-1, and it presented lower amounts of carbohydrates, when compared with other group. Total soluble proteins were statistically different among genotypes investigated , and was possible to show 3 groups. Higher protein level was obtained in SM 1007 genotype with 15.1 mg g DM-1. Additionally, CNFC 10408 genotype presented 12.8 mg g DM-1, and this value is statistically intermediate. In general, genotypes evaluated oscillated between 9.4 and 11.3 mg g DM-1.

CONCLUSÃO:

This experiment reveals that total soluble carbohydrates and total soluble proteins presented significant difference among genotypes evaluated. In addition, genotypes that presented higher carbohydrates and proteins levels were BRS Campeiro and SM 1007, respectively.

Palavras-chave: common bean, total soluble carbohydrates, total soluble proteins.