Female wistar rats (200-250g of body weight) with normal estrus cycle were used in our study. Firstly, we established the exercise protocol able to induce GH release. Thus, rats were submitted to  20 minutes of treadmill running at 75% of maximal aerobic capacity (MAC). To determine MAC, each rat performed an exercise test until exhaustion, initiating with 17 cm/s and increasing every 2 minutes (2cm/s) five days before experiment. Animals were killed by decapitation just after (0 min), 10, 20 and 30 min after running. Non-exercised animals stayed on the treadmill with minimal speed (5 cm/s). Serum GH analyses were done by radioimmunoassay (RGH - 45HK, LINCO, Missouri, USA). Brown adipose tissue (BAT) was dissected out and type2 iodothyonine deiodinase (D2) activity was measured. Pituitaries were processed in specific buffer to evaluate GH protein content by Western blotting. After that, we divided a new set of animals into four groups: non- and exercised sham operated, and non- and exercised ovariectomized (OVX) rats. Four days after the chirurgical procedures, MAC were determined. Ten days after surgery animals were submitted to the exercise session and were killed just after (0 min), 30 and 60 min after running. Non-exercised groups were used as baseline levels. Pituitaries were dissected out and type 1 iodothyronine deiodinase were measured. One-way analysis of variance was used to compare time points. The statistical significance was defined as p<0.05.